NANOTOXICOLOGY AND BIOINTERACTION ASSESSMENT OF BIOMEDICAL NANOMATERIALS
Bulgaria Bulgaria 
Indonesia 
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Biointeraction, Biomedical Nanomaterials, Cytotoxicity, Safety Assessment, Nanotoxicology
Abstract
Biomedical nanomaterials have garnered significant attention for their potential applications in medical diagnostics, drug delivery, and therapeutic interventions. However, concerns regarding their toxicity and biointeraction with biological systems remain largely unaddressed. Understanding the safety and biological interactions of these materials is crucial for ensuring their efficacy and safety in clinical settings. The aim of this study was to assess the nanotoxicological properties of biomedical nanomaterials and their interactions with biological systems. The research focused on evaluating the cytotoxicity, genotoxicity, and immunotoxicity of various nanomaterials commonly used in biomedical applications. A combination of in vitro and in vivo assays was employed to assess the toxicological profile of biomedical nanomaterials. These included cell viability tests, oxidative stress analysis, DNA damage assays, and immune response evaluations. The interactions between nanomaterials and cellular components were also examined using advanced imaging and spectroscopy techniques. The findings indicated that the toxicity of nanomaterials varied depending on their size, surface charge, and composition. Certain nanomaterials demonstrated significant cytotoxic and genotoxic effects, while others showed minimal toxicity. The biointeractions were also influenced by the concentration and exposure duration. The study underscores the need for comprehensive toxicity assessments of biomedical nanomaterials to ensure their safe application in medical technologies. Further research is required to optimize their safety profiles for clinical use.
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References
Ahmed, S. S. U., & Rahman, M. Z. (2024). 7.11—Metal- and metal oxide-based nanomaterials: From synthesis to applications. In S. Hashmi (Ed.), Comprehensive Materials Processing (Second Edition) (pp. 236–254). Elsevier. https://doi.org/10.1016/B978-0-323-96020-5.00282-X
Aljarba, N. H., Afzal, M., Alkhateeb, M. A., & Alkahtani, S. (2026). Functional 2D nanomaterial loaded biopolymer grafted semi-IPN hydrogel for enhanced neuroprotective drug release performance. Diamond and Related Materials, 161, 113194. https://doi.org/10.1016/j.diamond.2025.113194
Aslam, F., Guo, J., Khalid, A., Anwar, S., Arshad, K., Khan, M. N., Lai, P., & Liu, L. (2025). Carbon dots as probes in FLIM:a review of applications and advances in cellular imaging. RSC Advances, 15(52), 44919–44960. https://doi.org/10.1039/d5ra05371d
Bohlooli, M., Khajeh, M., Ghaffari-Moghaddam, M., & Pakdel, A. (2026). Quantitative design principles for biofunctional metal–organic frameworks: Stability thresholds, biointerface energetics, and therapeutic applications. Materials Today Bio, 38, 103166. https://doi.org/10.1016/j.mtbio.2026.103166
Chattopadhyay, D., & Das, B. (2025). Chapter 6—Role of the polymeric structure and nanocomposites in tissue engineering. In Design, Characterization and Fabrication of Polymer Scaffolds for Tissue Engineering (pp. 151–195). Elsevier Science Ltd. https://doi.org/10.1016/B978-0-323-96114-1.00011-2
Chen, H., Qiao, Y., Liu, J., Bian, D., Zhao, Y., Fan, X., Du, J., & Zhang, S. (2026). Thermoresponsive Chitosan Nanocomposite-Based Double-Network Hydrogel for Sustained Tumor Immunotherapy. Biomacromolecules. https://doi.org/10.1021/acs.biomac.5c02437
Congur, G., & Erdem, M. (2025). The development of biopolyol/chitosan modified single-use disposable electrochemical biosensor and its application for the voltammetric monitoring of the biointeraction between ziram and double stranded DNA. International Journal of Biological Macromolecules, 316, 144575. https://doi.org/10.1016/j.ijbiomac.2025.144575
Costa, S. M., Mattos, B. D., Calhelha, R. C., Zhu, Y., Lima, E., Reis, L. V., Rojas, O. J., Fangueiro, R., & Ferreira, D. P. (2025). Electrospun polycaprolactone membranes functionalized with nanochitin for enhanced bioactivity in localized cancer photodynamic therapy. Carbohydrate Polymer Technologies and Applications, 11, 100895. https://doi.org/10.1016/j.carpta.2025.100895
D?bkowska, M., Kosiorowska-Kraj, A., Szatanik, A., Filip, K., Martínez-Orts, M., Pujals, S., Olszewska, M., & Pukacka, K. (2026). Tunable PEGylated albumin nanocarriers enhance 5-FU cytotoxic selectivity and modulate oxidative and immune stress in colorectal cancer model. Biomedicine & Pharmacotherapy, 196, 118958. https://doi.org/10.1016/j.biopha.2025.118958
Dheyab, M. A., Abdullah, W., Abdulwahab, S., Alsarayreh, S. M., Tarawneh, M. H., Alsardi, M. M., Alanazi, M. A., & Abdul Aziz, A. (2025). Force-driven architectonics of inorganic nanomaterials: Pathways to smart and functional interfaces. RSC Mechanochemistry, 3(2), 161–190. https://doi.org/10.1039/d5mr00116a
Dilnawaz, F., Tripathy, N. S., Sahoo, L., Kumar Paikray, S., & Misra, A. N. (2026). Chapter 22—Halloysite nanotubes as emerging multifunctional materials for environmental and biomedical applications. In D. Rawtani, N. Khatri, & C. M. Hussain (Eds.), Smart Halloysite Nanotubes (pp. 429–449). Elsevier. https://doi.org/10.1016/B978-0-443-15912-1.00004-5
Duan, Y., Chen, S., Wang, Y., Liu, Z., Wei, L., Luo, B., Liu, W., & Gao, H. (2026). Chiral biomaterials for promoting wound healing: Fabrication strategies, therapeutic applications, and future prospects. Colloids and Surfaces B: Biointerfaces, 259, 115328. https://doi.org/10.1016/j.colsurfb.2025.115328
Estévez, M., Cicuéndez, M., Colilla, M., Vallet-Regí, M., González, B., & Izquierdo-Barba, I. (2024). Magnetic colloidal nanoformulations to remotely trigger mechanotransduction for osteogenic differentiation. Journal of Colloid and Interface Science, 664, 454–468. https://doi.org/10.1016/j.jcis.2024.03.043
Fakhar, A., Ahmed, M. A., Kim, N. Y., Kim, H. J., Kim, T. M., & Choi, J. W. (2025). Phenolated lignin nanoparticles with improved stability and biofunctionality: A comparative study of nanoprecipitation and solvent exchange fabrication techniques. International Journal of Biological Macromolecules, 332, 148724. https://doi.org/10.1016/j.ijbiomac.2025.148724
Fan, Y., Chen, L., Zhang, J., Liu, C., Liu, L., Luo, R., Xie, S., Li, Z., Liu, Y., & Luo, D. (2026). Black phosphorus-based nanomedicines. Matter, 9(3), 102634. https://doi.org/10.1016/j.matt.2025.102634
Feng, H., Hong, Y., Li, Q., & Qu, S. (2024). Advancements in research on the carbon dots nanomaterials in immune modulate and immunotherapy. Chemical Engineering Journal, 502, 157991. https://doi.org/10.1016/j.cej.2024.157991
García-Simarro, M. P., Mondéjar-López, M., Aguado, C., Ahrazem, O., Gómez-Gómez, L., & Niza, E. (2026). Carboxymethyl chitosan-cinnamaldehyde coated dendritic silica hybrid nanoparticles: A new improved antifungal agent for seed treatment through dual release of terpenes. Plant Nano Biology, 15, 100242. https://doi.org/10.1016/j.plana.2025.100242
Hu, W., Qian, X., Lin, X., Chen, Q., Wu, Z., Chen, Z., Xue, Z., Chen, Y., Xu, X., & Luo, K. (2025). Zeolitic imidazolate frameworks exert bioenergetic modulation via the cAMP/PKA/CREB signaling pathway to accelerate periodontal regeneration. Chemical Engineering Journal, 526, 171251. https://doi.org/10.1016/j.cej.2025.171251
Hussain, Z., Ahmed, M. N., Jagal, J., Rawas-Qalaji, M., & Tarazi, H. (2025). Dual targeting of prostate cancer cells and tumor-associated macrophages for mitigating tumorigenesis and metastasis: Hyaluronic acid functionalized polymeric nanospheres for CD44-mediated active targeting. Journal of Molecular Liquids, 434, 128025. https://doi.org/10.1016/j.molliq.2025.128025
Kumari, N. U., Chigurupati, S. P. D., Rajana, N., Vasave, R., Bahadure, S., & Mehra, N. K. (2026). Pre-programming the protein corona: From avoidance to endogenous targeting. Journal of Controlled Release, 389, 114447. https://doi.org/10.1016/j.jconrel.2025.114447
Mansour, H., Okba, E. A., Ibrahim, M. M., Elshami, F. I., & Shaban, S. Y. (2025). A kinetic and mechanistic study of chitosan-functionalized lanthanum zinc ferrite nanoparticles: Balancing biomolecular affinity with anticancer, antibacterial, and antioxidant functions. Inorganic Chemistry Communications, 181, 115230. https://doi.org/10.1016/j.inoche.2025.115230
Minh Hoang, C. N., Nguyen, S. H., & Tran, M. T. (2025). Nanoparticles in cancer therapy: Strategies to penetrate and modulate the tumor microenvironment – A review. Smart Materials in Medicine, 6(2), 270–284. https://doi.org/10.1016/j.smaim.2025.07.004
Ogungbesan, S. O., Etafo, N. O., Anselm, O. H., Ejeromedoghene, O., Kalulu, M., Abdullah, M., Diaz, D. D., & Fu, G. (2025). Transition metal oxide nanohybrid materials: A review of their structures, properties, and applications. Journal of Molecular Structure, 1337, 142209. https://doi.org/10.1016/j.molstruc.2025.142209
Oisakede, E. O., Oyedeji, O. O., Olawuyi, O. F., Alabi, J. O., Daniel, R. I. A., & Olawade, D. B. (2026). Nanoparticle-mediated cardiotoxicity and nanomedicine interventions in cancer treatment. Nano TransMed, 5, 100113. https://doi.org/10.1016/j.ntm.2026.100113
Pareek, A., Alasiri, G., Dudhwala, Y., Alaseem, A. M., Alsaidan, O. A., Kapoor, D. U., & Prajapati, B. G. (2025). Review of engineered magnetic chitosan nanoparticles for drug delivery: Advances, challenges, and future prospects. International Journal of Biological Macromolecules, 327, 147441. https://doi.org/10.1016/j.ijbiomac.2025.147441
Parmar, N. B., Desai, M. D., Mehta, K. N., Chorawala, M. R., Prajapati, B. G., Patel, R. B., & Kote, P. C. (2026). Chapter 11—Biocompatibility and safety considerations of nanodots. In B. G. Prajapati, D. U. Kapoor, & N. Ali (Eds.), Nanodots for Cancer Diagnosis and Treatment (pp. 257–302). Academic Press. https://doi.org/10.1016/B978-0-443-27511-1.00011-X
Sabarees, G., Sam Jebaraj, Y., Ezhilarasan, E., & Dravid Ragul, Y. (2026). Next-generation injectable hydrogels: Advanced crosslinking strategies, multi-stimuli responsiveness, and translational advances for precision regenerative medicine. Nano TransMed, 5, 100109. https://doi.org/10.1016/j.ntm.2025.100109
Scapolan, M. I. X., Nicoletti, M. A. G., de Souza, P. R., Faria, L. M. de L., Feitosa, E., Martins, A. F., & Adati, R. D. (2025). Chitosan/alginate-based layer-by-layer films with europium ions as anti-adhesive luminescent coatings. Surfaces and Interfaces, 78, 108159. https://doi.org/10.1016/j.surfin.2025.108159
Sekaran, S., Raju, L., & Eswaramoorthy, R. (2025). Chapter 4—Biointeraction of nanomaterials with marine biopolymers. In S. Ahmed & A. Soundararajan (Eds.), Marine Biopolymers (pp. 105–123). Elsevier. https://doi.org/10.1016/B978-0-443-15606-9.00004-8
Seo, G., Kim, B., Lim, H., Choi, J., Kim, M., Lee, H., & Kim, H.-O. (2025). Biomedical applications and future perspectives of carbon dots and their hybrid nanomaterials. Materials Advances, 7(1), 157–174. https://doi.org/10.1039/d5ma00816f
Shahid, S. A., Ijaz, S., Iqbal, J., Khalil, A. T., & Ovais, M. (2024). Chapter 10—Safety considerations of organic nanomaterials for phototheranostics. In M. Abbas, A. Atiq, M. Ovais, & M. R. Hamblin (Eds.), Organic Nanomaterials for Cancer Phototheranostics (pp. 233–252). Woodhead Publishing. https://doi.org/10.1016/B978-0-323-95758-8.00007-1
Sojitra, S. C., Mishra, S. R., Patel, D., Shah, P. A., Sharma, V., & Shrivastav, P. S. (2025). Chapter 7—Biosensors used for minimally invasive drug delivery monitoring. In M. S. Hasnain, A. K. Nayak, & T. M. Aminabhavi (Eds.), Applications of Biosensors in Healthcare (pp. 103–162). Academic Press. https://doi.org/10.1016/B978-0-443-21592-6.00010-0
Song, Y. H., Chakraborty, G., Mahata, M. K., & De, R. (2024). Chapter 25—Functionalized nanomaterials: Health and safety. In H. Barabadi, E. Mostafavi, & C. Mustansar Hussain (Eds.), Functionalized Nanomaterials for Cancer Research (pp. 561–577). Academic Press. https://doi.org/10.1016/B978-0-443-15518-5.00016-1
Tade, R. S., & Pawara, D. L. (2025). Synthesis, properties and toxicological perspectives of few-layered black phosphorus and black phosphorus quantum dots: A review. Inorganic Chemistry Communications, 174, 114077. https://doi.org/10.1016/j.inoche.2025.114077
Timochenco, L., Fernandes, P. D., Ribeirinho-Soares, S., Silva, F. A. L. S., Freitas, B., Nunes, O. C., Oliveira, M. J., Magalhães, F. D., & Pinto, A. M. (2025). Long-term study of physicochemical stability, microbial contamination, and endotoxin levels in UVC-photoreactor sterilized graphene-based materials. Carbon, 243, 120538. https://doi.org/10.1016/j.carbon.2025.120538
Trivedi, R., Malode, D., Umekar, M., Shidhaye, S., Khobragade, R., & Raut, N. (2026). Graphene quantum dots: Synthesis, applications, and future directions in bioimaging and cancer therapy. Next Nanotechnology, 9, 100326. https://doi.org/10.1016/j.nxnano.2025.100326
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Copyright (c) 2026 Muntasir Muntasir, Ivan Dimitrov, Maria Ivanova
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